Volume 15, Issue 58 (4-2025)
NCMBJ 2025, 15(58): 71-80 |
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Pourbabaee F, Siasi E, Rafiei Tabatabaii R. Study of association between SHARPIN gene rs34173062 polymorphism in and Alzheimer’s disease by Tetra ARMS PCR. NCMBJ 2025; 15 (58) :71-80
URL: http://ncmbjpiau.ir/article-1-1742-en.html
URL: http://ncmbjpiau.ir/article-1-1742-en.html
Department of Genetic, School of science, Islamic Azad University, North Tehran Branch, Tehran, Iran. , emi_biotech2006@yahoo.ca
Abstract: (250 Views)
Aim and Background: However, the molecular mechanisms of Alzheimer's disease are unknown. Various genetic and environmental factors can cause Alzheimer's disease and are used to diagnose and predict the disease. The aim of this study was to investigate the presence of rs34173062 polymorphism in the SHARPIN gene and its association with triggering Alzheimer's disease in the Iranian patient population.
Materials and Methods: This case-control study was conducted in 2021 on 50 people with Alzheimer's disease and 50 healthy controls. After DNA extraction from blood samples, genotyping was performed using the Tetra-ARMS PCR method and the data were statistically analyzed.
Results: The genotype results for rs34173062 polymorphism showed that the frequencies of AA, AG and GG genotypes in the patient group were 62%, 10% and 28%, respectively, and in the control group were 48%, 18% and 34%, respectively. There was no significant association between rs34173062 polymorphism and the risk of Alzheimer's disease (P=0.313). Among the demographic factors studied, only the association between age and affected disease was significant (P=0.029).
Conclusion: The results showed that there was no statistically significant association between the rs34173062 polymorphism of the SHARPIN gene and Alzheimer's disease in the Iranian population studied. To confirm the results of the present study, it is recommended to study populations from different societies and a larger number of samples.
Materials and Methods: This case-control study was conducted in 2021 on 50 people with Alzheimer's disease and 50 healthy controls. After DNA extraction from blood samples, genotyping was performed using the Tetra-ARMS PCR method and the data were statistically analyzed.
Results: The genotype results for rs34173062 polymorphism showed that the frequencies of AA, AG and GG genotypes in the patient group were 62%, 10% and 28%, respectively, and in the control group were 48%, 18% and 34%, respectively. There was no significant association between rs34173062 polymorphism and the risk of Alzheimer's disease (P=0.313). Among the demographic factors studied, only the association between age and affected disease was significant (P=0.029).
Conclusion: The results showed that there was no statistically significant association between the rs34173062 polymorphism of the SHARPIN gene and Alzheimer's disease in the Iranian population studied. To confirm the results of the present study, it is recommended to study populations from different societies and a larger number of samples.
Type of Study: Research Article |
Subject:
Cellular and molecular
Received: 2025/04/28 | Accepted: 2025/04/3 | Published: 2025/04/3
Received: 2025/04/28 | Accepted: 2025/04/3 | Published: 2025/04/3
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