Volume 16, Issue 61 (3-2026)
NCMBJ 2026, 16(61): 83-100 |
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Amraei B. Selective cytotoxicity and apoptosis induction by biosynthesized silver nanoparticles using )Serenoa repens( extract in breast and prostate cancer cell lines. NCMBJ 2026; 16 (61) :83-100
URL: http://ncmbjpiau.ir/article-1-1801-en.html
URL: http://ncmbjpiau.ir/article-1-1801-en.html
Assistant Professor, Department of Biology, Payame Noor University, Tehran, Iran;
Abstract: (86 Views)
Aim and Background: Silver nanoparticles (AgNPs) are known for their anticancer properties, while Serenoa repens (Saw Palmetto) extract has demonstrated inhibitory effects on cancer cell growth. This study investigates the selective cytotoxicity of AgNPs synthesized with S. repens extract on breast (MCF-7) and prostate (PC-3) cancer cell lines, compared to normal cells (MCF-10A and RWPE-1).
Materials and Methods: AgNPs were synthesized using an aqueous extract of S. repens. Cell viability was assessed at 24, 48, and 72 hours using the MTT assay. Apoptosis and cell death were analyzed through Annexin V/PI staining. Cells were treated with 10–80 µg/mL of AgNPs, and IC50 values were calculated. Statistical analyses were used to compare group differences.
Results: The synthesized AgNPs exhibited dose-dependent cytotoxic effects on MCF-7 and PC-3 cells, with IC50 values of 25 and 50 μM, respectively. Linear regression analysis confirmed a significant decrease in cancer cell viability with increasing nanoparticle concentrations, while minimal effects were observed on normal cell lines (p<0.05).
Conclusion: AgNPs synthesized with S. repens extract selectively target cancer cells while sparing normal cells, indicating high therapeutic potential against breast and prostate cancers. Further studies are needed to determine optimal dosing and clarify apoptotic mechanisms.
Materials and Methods: AgNPs were synthesized using an aqueous extract of S. repens. Cell viability was assessed at 24, 48, and 72 hours using the MTT assay. Apoptosis and cell death were analyzed through Annexin V/PI staining. Cells were treated with 10–80 µg/mL of AgNPs, and IC50 values were calculated. Statistical analyses were used to compare group differences.
Results: The synthesized AgNPs exhibited dose-dependent cytotoxic effects on MCF-7 and PC-3 cells, with IC50 values of 25 and 50 μM, respectively. Linear regression analysis confirmed a significant decrease in cancer cell viability with increasing nanoparticle concentrations, while minimal effects were observed on normal cell lines (p<0.05).
Conclusion: AgNPs synthesized with S. repens extract selectively target cancer cells while sparing normal cells, indicating high therapeutic potential against breast and prostate cancers. Further studies are needed to determine optimal dosing and clarify apoptotic mechanisms.
Keywords: Silver Nanoparticles, Serenoa Repens, Breast Cancer, Prostate Cancer, Apoptosis, Selective Cytotoxicity
Type of Study: Research Article |
Subject:
Cellular and molecular
Received: 2026/03/12 | Accepted: 2026/03/1 | Published: 2026/03/1
Received: 2026/03/12 | Accepted: 2026/03/1 | Published: 2026/03/1
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