Volume 3, Issue 10 (6-2013)                   NCMBJ 2013, 3(10): 15-20 | Back to browse issues page

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Ranji, N, Sadeghizadeh M, Karimipour M, Shokrgozar M. Investigation of let-7 family expression in CD4+ T cells induced by anti-CD2, CD3, CD28 and Interleukin-2 and identifying their potential targets . NCMBJ. 2013; 3 (10) :15-20
URL: http://ncmbjpiau.ir/article-1-328-en.html
Department of Biology, Science and Research Branch, Islamic Azad University,Tehran, Iran , najmehranji@gmail.com
Abstract:   (22760 Views)

Aime and Background. The aim of this study was to evaluate let-7 family members expression in activated naïve CD4+ T cells and IL-2 induced cells. Let-7 family has oncogenic and tuomor suppressive activity in different cells. 

Methods. Naïve CD4+ T cells were activated by anti-CD2, CD3, CD28 microbeads. After 3 days, the activated cells stimulated by IL-2(0.75ng/ml). after 72h total RNA extracted the cells and c-DNA synthesis and Q-RT-PCR performed by miRCURY LNATM universal RT miRNA PCR kit. miRNA expression analyzed by geneX software and ∆∆Ct method.

Results. Five members of let-7 family over-expressed in activated cells compared with IL-2 induced cells. Targets Predicted by miRDB and miRanda software including families of DUSP, PTPN and SOCS that two first families inhibit TCR signaling and latest suppress JAK/STAT pathway.

Conclusion. It appears that let-7 family play important role in clonal expansion of activated CD4+ T cells by specific genes suppression in post-transcriptional levels for appropriated immune response.

Full-Text [DOC 38 kb]   (4029 Downloads)    
Type of Study: Research Article | Subject: Immonology
Received: 2013/05/26 | Published: 2013/06/15

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