Aim and Background : Aloe vera is one of the important medicinal plants, that grow in warm-dried regions. In this study, fifty Aloe vera populations from five different areas from Iran, were evaluated for their morphological traits.

Materials and methods : During of growing season 18 morphological characters such as height, breadths of plant, number of leaves, length and width of leaf and were measured. Data was analyzed using SPSS software.

Results : The results showed that some characters had positive correlation. Leaf length with width of leaf had positive significant correlation at 5% level of significance. Borazjan population had the highest weight leaf (262 g), diameter leaf (2.5 cm). Bashagerd population had highest weight plant (18Kg) and flower length (120cm).

Conclusion : Cluster analysis resulted in the populations being divided into three groups. Results indicated that, Iranian Aloe vera is completely different from the common aloe species with diversity that makes it a valuable genetic resource for breeding programs.