Volume 5, Issue 19 (7-2015)
NCMBJ 2015, 5(19): 53-60 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Yazdansetad S, Taheri R, Ajoudanifar H. Production of microbial cellulose by native species of Pseudomonas luteola. NCMBJ 2015; 5 (19) :53-60
URL: http://ncmbjpiau.ir/article-1-650-en.html
URL: http://ncmbjpiau.ir/article-1-650-en.html
Young Researchers and Elite Club, Damghan Branch, Islamic Azad University, Damghan, Iran , sajjad.yazdansetad@gmail.com
Abstract: (7249 Views)
Aims and Background: Cellulose is the most widely used biopolymer to produce paper, textiles and biomedical materials. One of the interesting methods to produce cellulose is microbial resource due to the shortage of cellulose original resources. On the other hand, the use of microbial cellulose as a pure polymer and absence of lignin and hemicellulose is preferred. The present study was aimed to isolate Pseudomonas luteola producing cellulose bacterium to produce cellulose and evaluate its production quality.
Materials and Methods: Samples from different local vinegars, juices and fruits were cultured in Hestrin-Schramm agar medium. Pseudomonas luteola was isolated with biochemical tests and verified by 16S rRNA typing method. After cultivation of the isolate in Hestrin-Schramm broth, cellulose was produced in medium. The cellulose layer was treated by SDS and NaOH, then purified and dried. Finaly, the produced cellulose was verified by cellulase enzyme and scaning electron microscope (SEM).
Results: In this study, the gram-negative organism Pseudomonas luteola was isolated and identified. Then, the isolate was cultured in Hestrin-Schramm broth and cellulose was produced after 6 days incubation at 30°C. The enzymatic digestion results were showed that the cellulose has been presented in culture medium. Scaning Electron Microscope (SEM) image was revealed that the produced cellulose by the isolate has fibrous and plexiform structure.
Conclusion: This is the first report on cellulose production by native isolate of Pseudomonas luteola in Iran. The quality of produced cellulose by the organism is appropriate in comparison with the cellulose produced by standard strain, thus is presented to produce cellulose as a new resource.
Materials and Methods: Samples from different local vinegars, juices and fruits were cultured in Hestrin-Schramm agar medium. Pseudomonas luteola was isolated with biochemical tests and verified by 16S rRNA typing method. After cultivation of the isolate in Hestrin-Schramm broth, cellulose was produced in medium. The cellulose layer was treated by SDS and NaOH, then purified and dried. Finaly, the produced cellulose was verified by cellulase enzyme and scaning electron microscope (SEM).
Results: In this study, the gram-negative organism Pseudomonas luteola was isolated and identified. Then, the isolate was cultured in Hestrin-Schramm broth and cellulose was produced after 6 days incubation at 30°C. The enzymatic digestion results were showed that the cellulose has been presented in culture medium. Scaning Electron Microscope (SEM) image was revealed that the produced cellulose by the isolate has fibrous and plexiform structure.
Conclusion: This is the first report on cellulose production by native isolate of Pseudomonas luteola in Iran. The quality of produced cellulose by the organism is appropriate in comparison with the cellulose produced by standard strain, thus is presented to produce cellulose as a new resource.
Type of Study: Research Article |
Subject:
Cellular and molecular
Received: 2015/07/15 | Accepted: 2015/07/15 | Published: 2015/07/15
Received: 2015/07/15 | Accepted: 2015/07/15 | Published: 2015/07/15
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
