Volume 5, Issue 20 (10-2015)
NCMBJ 2015, 5(20): 23-28 |
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Naseri F, Daemii M R, Sangtarash M H, Torkamanzehi A. Detection and Comparison of Sequences of VP28 Gene of White Spot Syndrome Virus Affecting Litopenaeus vannamei in Chabahar (Iran). NCMBJ 2015; 5 (20) :23-28
URL: http://ncmbjpiau.ir/article-1-695-en.html
URL: http://ncmbjpiau.ir/article-1-695-en.html
university of sistan and balouchestan , Fnaseri55@gmail.com
Abstract: (9078 Views)
Aim and Background: White spot syndrome virus (WSSV) infection has resulted in severe production and economic losses in shrimp culture globally.
The first time the white spot disease (WSD) was reported in Iran was in 2004 and 2008, respectively in Bushehr Province. In Sistan and Balochestan province only the WSD was reported in 2008. White spot syndrome virus (WSSV) is the most pathogenic among the penaeid shrimp viruses.
Materials and Methods: WSSV DNA samples for PCR test were prepared from diseased shrimp. Specific PCR primers were used for the amplification of VP28 gene in WSSV. VP28 gene of DNA isolate of WSSV was amplified by PCR and the PCR product was sequenced.
Results: The results were submitted to GenBank (KF723558). The nucleotide sequence (KF723558) was compared with the VP28 gene sequences in the GenBank database (NCBI).
Conclusion: 36 sequences showed significant alignments with 99% homology which include the sequences from Korea, Japan, US, Indonesia, China, Vietnam, Taiwan, the Netherlands and Thailand. However, the homology was not observed 100% in the sequences.
Materials and Methods: WSSV DNA samples for PCR test were prepared from diseased shrimp. Specific PCR primers were used for the amplification of VP28 gene in WSSV. VP28 gene of DNA isolate of WSSV was amplified by PCR and the PCR product was sequenced.
Results: The results were submitted to GenBank (KF723558). The nucleotide sequence (KF723558) was compared with the VP28 gene sequences in the GenBank database (NCBI).
Conclusion: 36 sequences showed significant alignments with 99% homology which include the sequences from Korea, Japan, US, Indonesia, China, Vietnam, Taiwan, the Netherlands and Thailand. However, the homology was not observed 100% in the sequences.
Type of Study: Research Article |
Subject:
Genetics
Received: 2015/10/22 | Accepted: 2015/10/22 | Published: 2015/10/22
Received: 2015/10/22 | Accepted: 2015/10/22 | Published: 2015/10/22
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