Aim and Background: Chromosomal information of different species, in addition to improving classification and biological recognitions; it can be effective in breeding and genetic studies.

Material and Methods: Bred stocks were preyed from the east and west of Jask Harbour. Different growth stages were stained by Cholchicine and hypotonic KCL in different concentration and duration. Metaphase chromosome spreads were studied based on Campos-Ramos and Crash tissue methods and stained by Giemsa and distilled water.

Results: The testis tissue showed the best obtained spread. The number of diploid chromosomes (2n=86) was confirmed by the modal haploid chromosome number (n=43) in which 27 and 16 chromosome pairs were counted in meta/submetacentric and acro/telocentric, respectively.

Discussion: despite observed chromosome variations in karyotyping further Penaeidae family, the achieved diploid chromosome number of this study (2n= 86) exits within the range of diploid chromosome numbers previously reported for other species of shrimp (86 to 92).

Conclusion: treatments of testis tissue, staining by Giemsa 10% and crashing tissue method recognized as the best methods for karyotyping and chromosome spread analysis of Penaeidae family.