Aim and Background: Chemical mutagen mutagenic effects, diverse gene pool in plant resources and as a complementary tool in breeding programs are used. In this study, in order to make changes and changes in the morphological characteristics of different concentrations of active ingredients in the plant peppermint ethyl methane sulfonate were used EMS in vitro and field.

­­Material and Methods: Research in vitro and farm with 4 treatments and 4 replications in a completely randomized design. From different treatments EMS includes 0/0% (control group), 0/05% and 0/01% and 0­/­005% and for different times (24 and 48) and the field was used in vitro. Evaluation was conducted in a test tube and simultaneously farm. After the transfer and development of subjects in conditions in vitro, treatment with the mutagen ethyl methane sulfonate were studied.

­­Conclusion: The results showed that the use of MS medium mutagenic substances in medicinal plants play a role in phenotypic variation. Concentration of 0/01% EMS best result was significant at 1% and cultured explants were obtained, including that on the lateral buds and trailing characters such as stem length, number of leaves and buds of the side effect. The result analysis showed that the ethyl methane sulfonate oil treatments on materials essential components have not been affected.

Results: Use mutagen EMS in peppermint plant tissue culture and in both instances treated plants on a farm stimulate the rapid growth of lateral buds of the plant, which increases the level of plant and thus increase the yield. Concentrations of greater than 0/05% EMS at 24 and 48 hours in loss of tissue samples and the field.