Volume 14, Issue 55 (6-2024)
NCMBJ 2024, 14(55): 83-92 |
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Momeni K, Ghorbian S, Ahmadpour E, Sharifi R. Decreased expression of genes involved in keratin synthesis in patients with cutaneous leishmaniasis. NCMBJ 2024; 14 (55) :83-92
URL: http://ncmbjpiau.ir/article-1-1685-en.html
URL: http://ncmbjpiau.ir/article-1-1685-en.html
Decreased expression of genes involved in keratin synthesis in patients with cutaneous leishmaniasis
Department of Molecular Genetics, Ahar Branch, Islamic Azad University, Ahar, Iran , ghorbian20@gmail.com
Abstract: (670 Views)
Aim and Background: In many countries, including Iran, cutaneous leishmaniasis is a common and increasing disease. Proper and quick treatment of many of these patients is very difficult due to insufficient familiarity with the factors that play a role in the pathogenesis of cutaneous leishmaniasis. By using high-performance RNA sequencing methods and investigating the expression of different genes in different tissues, a new horizon has been opened on the identification of disease pathogenesis factors and obtaining new treatment methods.
Material and Methods: This investigation was divided into two phases. Data from the NCBI website, "GSE127831" were used for the bioinformatics analysis. We conducted investigations of Gene Ontology and Differential Gene Expression. In the clinical investigation, we used qPCR to assess the expression of the genes identified in the previous stage in 27 skin samples taken from wounds caused by cutaneous leishmaniasis and 12 healthy skin samples.
Results: According to our bioinformatics investigations, the first 100 genes with the highest drop in expression in leishmaniasis wounds are mostly involved in the production of different keratin proteins. They are mostly involved in "Intermediate Filaments Organization". The qPCR test showed that the expression of KRTAP11-1, KRT33B, KRT85, KRT35, KRT86, and KRT81 genes was greatly reduced. It shows the coordination of the results of bioinformatics and laboratory stages.
Conclusion: The downregulation of a specific set of genes involved in keratin formation is one of the key factors contributing to the poor healing of wounds in cutaneous leishmaniasis. keratin is a crucial chemical in developing and maintaining epithelial tissue and skin.
Material and Methods: This investigation was divided into two phases. Data from the NCBI website, "GSE127831" were used for the bioinformatics analysis. We conducted investigations of Gene Ontology and Differential Gene Expression. In the clinical investigation, we used qPCR to assess the expression of the genes identified in the previous stage in 27 skin samples taken from wounds caused by cutaneous leishmaniasis and 12 healthy skin samples.
Results: According to our bioinformatics investigations, the first 100 genes with the highest drop in expression in leishmaniasis wounds are mostly involved in the production of different keratin proteins. They are mostly involved in "Intermediate Filaments Organization". The qPCR test showed that the expression of KRTAP11-1, KRT33B, KRT85, KRT35, KRT86, and KRT81 genes was greatly reduced. It shows the coordination of the results of bioinformatics and laboratory stages.
Conclusion: The downregulation of a specific set of genes involved in keratin formation is one of the key factors contributing to the poor healing of wounds in cutaneous leishmaniasis. keratin is a crucial chemical in developing and maintaining epithelial tissue and skin.
Keywords: Bioinformatics Analysis, Differential gene expression analysis, Gene Ontology Analysis, Cutaneous leishmaniasis, Keratin, Iau Science.
Type of Study: Research Article |
Subject:
Physiology
Received: 2024/08/18 | Accepted: 2024/06/30 | Published: 2024/06/30
Received: 2024/08/18 | Accepted: 2024/06/30 | Published: 2024/06/30
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