Charactrization and isolation of Nocardia from clinical sample Patient suspected to active tuberculosis

Zaker Bostanabad, saeed; Hashemi-Shahraki, abdolrazzagh; Heidarieh, parvin; Sheikhi, nasrin; Sharifi, arman; Seyri Fouron Abad, masoumeh

Volume 4, Issue 13 (3-2014) NCMBJ 2014, 4(13): 19-23 | Back to browse issues page

‎ 20.1001.1.22285458.1392.4.13.3.2

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Zaker Bostanabad S, Hashemi-Shahraki A, Heidarieh P, Sheikhi N, Sharifi A, Seyri Fouron Abad M. Charactrization and isolation of Nocardia from clinical sample Patient suspected to active tuberculosis. NCMBJ 2014; 4 (13) :19-23
URL: http://ncmbjpiau.ir/article-1-474-en.html

Charactrization and isolation of Nocardia from clinical sample Patient suspected to active tuberculosis

Saeed Zaker Bostanabad

, Abdolrazzagh Hashemi-Shahraki

, Parvin Heidarieh

, Nasrin Sheikhi

, Arman Sharifi

, Masoumeh Seyri Fouron Abad

Islamic Azad University, Parand branch, Tehran, Iran , saeedzaker20@yahoo.com

Abstract: (14347 Views)

Background and Aim: Nocardia as one of the most important genera of aerobic actinomycetes, soil dwellers are that in some cases, causing dangerous and lethal infections in susceptible patients, especially patients with various immune deficiencies. A number of species belonging to Nocardia can be cause chronic pulmonary infections. In this study Nocardiosis in patients with suspected pulmonary tuberculosis in patients who were negative for culture and microscopic slides, were examined.

Material and Method: 90 patients suspected of Mycobacterium tuberculosis with negative sputum re-provision and Nocardia isolated as pathogens were studied. Paraffin baiting technique and cultured on Blood agar medium was used for isolation of Nocardia isolates. Clinical Isolates were identified by microbiological phenotypic methods identification were determined.

Result: Six clinical isolates of Nocardia from Tuberculosis patients with negative cultures and smears were isolated.

Conclusion: In patients with suspected tuberculosis, which are detected by culture and negative microscopic, Nocardia as a cause of chronic lung disease can be important and should be considered.

Keywords: Tuberculosis, Negative culture, Nocardia, Characterization

Full-Text [PDF 6648 kb] (3171 Downloads)

Type of Study: Research Article | Subject: Cellular and molecular
Received: 2014/04/20 | Accepted: 2014/04/20 | Published: 2014/04/20