Volume 8, Issue 30 (4-2018)
NCMBJ 2018, 8(30): 71-78 |
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Sajadian M, Doudi M, Shirmardi A. Isolation and molecular identification of resistant bacteria Pb from soils oil contaminated in area C-branch Masjed Soleiman city in Khuzestan . NCMBJ 2018; 8 (30) :71-78
URL: http://ncmbjpiau.ir/article-1-1083-en.html
URL: http://ncmbjpiau.ir/article-1-1083-en.html
Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran. , Monirdoudi@yahoo.com
Abstract: (6591 Views)
Aim and Background:
Due to the high prevalence of diseases caused by the presence of lead in soil contaminated with petroleum industrial centers, then transfer the metal to plants and animals and eventually humans and high risks for human health and other living things. It seems that identify and isolate microorganisms with high strength steel and the biological removal can be an important step in the evolution of contaminated soil cleanup systems considered industrial centers.
Material and methods:
The was sampling of three areas of oil contaminated soils C-branch Masjed Soleiman. To isolate resistant bacteria lead ΙΙ PHG agar dilution method and the culture medium containing concentrations of 0.5, 1, 2, 2.5, 3, 3.5 and 4 mMol per liter of lead were used. To identify of resistant bacteria lead , first was used of biochemical tests and then molecular methods PCR- colony.
Results:
In this study, 5 resistant bacteria lead from oil contaminated soils were isolated and identified that among of them Proteus mirabilis strain AOUC-001 bacteria and showed maximum resistance to lead (MIC=4mM).
Discussion and Conclusion:
According to the bacteria examined in this study could lead bioabsorption in -vitro and growth rather appropriate in the presence of the metal showed, so it seems a good candida for bioremedation of lead from oil contaminated soils in the future. Provided that the contaminated soils to isolate the bacteria to be filtered out treatment.
Due to the high prevalence of diseases caused by the presence of lead in soil contaminated with petroleum industrial centers, then transfer the metal to plants and animals and eventually humans and high risks for human health and other living things. It seems that identify and isolate microorganisms with high strength steel and the biological removal can be an important step in the evolution of contaminated soil cleanup systems considered industrial centers.
Material and methods:
The was sampling of three areas of oil contaminated soils C-branch Masjed Soleiman. To isolate resistant bacteria lead ΙΙ PHG agar dilution method and the culture medium containing concentrations of 0.5, 1, 2, 2.5, 3, 3.5 and 4 mMol per liter of lead were used. To identify of resistant bacteria lead , first was used of biochemical tests and then molecular methods PCR- colony.
Results:
In this study, 5 resistant bacteria lead from oil contaminated soils were isolated and identified that among of them Proteus mirabilis strain AOUC-001 bacteria and showed maximum resistance to lead (MIC=4mM).
Discussion and Conclusion:
According to the bacteria examined in this study could lead bioabsorption in -vitro and growth rather appropriate in the presence of the metal showed, so it seems a good candida for bioremedation of lead from oil contaminated soils in the future. Provided that the contaminated soils to isolate the bacteria to be filtered out treatment.
Keywords: Heavy metal resistant bacteria, Pb, Oil contaminated soils, Molecular identification, Minimum inhibitory concentration (MIC)
Type of Study: Research Article |
Subject:
Microbiology
Received: 2018/04/23 | Accepted: 2018/04/23 | Published: 2018/04/23
Received: 2018/04/23 | Accepted: 2018/04/23 | Published: 2018/04/23
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