Volume 8, Issue 32 (10-2018)                   NCMBJ 2018, 8(32): 65-72 | Back to browse issues page

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Teimoori P, Yahyaabady S, doudi M. Antimicrobial effect of Mentha Longifulia on different bacteria causing diarrhea and its phytochemical evaluation. NCMBJ. 2018; 8 (32) :65-72
URL: http://ncmbjpiau.ir/article-1-1145-en.html
Department of Microbiology, Islamic Azad Univercity of Falavarjan,Falavarjan-Iran , yahyaabadisima@gmail.com
Abstract:   (3296 Views)

Aim and Background:Increasing resistance to antibiotics and their harmful side effects have made it necessary to use alternative therapies such as plant compounds. In this research, the effects of various concentrations of wild Oregano essential oil were investigated on some bacteria(isolated from patients in the Isfahan Healthcare Network) responsible for diarrhea.
Material and methods:A total of 250 bacterial isolates were collected from urban, rural and hospital health centers of Isfahan Province of Iran and the bacterial  growth inhibitory effect of concentrations of 5, 10, 20 and 40% of wild oregano(Mentha Longifulia) essential oil (prepared  under drying conditions in shade, sunshine and microwave) was studied on these isolates by disk diffusion method in agar using Kirby-Bayer method
Results:All concentrations of oregano(Mentha Longifulia)   essential oils dried  under  shadow ,had a significant inhibitory effect on the growth of bacterial isolates. The most inhibitory effect was obtained at concentration of 40%.The most MIC and MBC Value of essential oils was determined at 20% and 40% of essential oils respectively. The highest isolated phytochemical was Thymol  with the amount of 37.99%, and the lowest was Alha-Terpinolene and E Z-Allo with 181.0%.
Conclusion:Antimicrobial properties of  Wild Oregano (Mentha Longifulia)  essential  oils is related to its polyphenolic compounds. The antibacterial properties of this plant, due to the high amount of thymol, can be partly related to this chemical composition. From the findings, it was concluded that the highest inhibitory concentration of Wild Oregano (Mentha Longifulia)  essential oil under drying in the shade was belonged to concentration of 40% and the highest isolated phytochemical was related to Thymol.

 
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Type of Study: Research Article | Subject: Cellular and molecular
Received: 2018/10/15 | Accepted: 2018/10/15 | Published: 2018/10/15

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