Volume 9, Issue 35 (6-2019)
NCMBJ 2019, 9(35): 25-34 |
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Goodarzi L, Yari R, Mirzaei M. dentifying antibiotic resistance genes in clinical isolates of Klebsiella pneumoniae producing IMP-1 and TEM β-lactamase. NCMBJ 2019; 9 (35) :25-34
URL: http://ncmbjpiau.ir/article-1-1201-en.html
URL: http://ncmbjpiau.ir/article-1-1201-en.html
Department of Biology, Borujerd Branch, Islamic Azad University, Borujerd, Iran
Abstract: (3682 Views)
Aim &Background: Klebsiella pneumoniae is a common cause of β-lactamase-associated infections in hospitals. The present study aimed to determine the frequency of antibiotic resistance genes in Klebsiella pneumoniae strains producing IMP-1 and TEM β-lactamase.
Materials and methods: The present research identified 94 samples of K. pneumoniae, using antibiogram for the phenotypic confirmation of ESBLs. The antibiotic resistance of the isolates and the prevalence of TEM and IMP-1 genes were determined using PCR method.
Findings: Of 94 samples, 77.6% were ESBL-positive and 22.3% ESBL-negative. A total of 4.1% of the samples carried the IMP-1 gene and 43.8% the TEM gene, while 43.8% of the samples carried both genes.
Conclusion: Given that TEM and IMP-1 genes were commonly present in a large number of the resistant samples, physicians are recommended to use therapeutic measures properly, and to prescribe antibiotics rationally.
Materials and methods: The present research identified 94 samples of K. pneumoniae, using antibiogram for the phenotypic confirmation of ESBLs. The antibiotic resistance of the isolates and the prevalence of TEM and IMP-1 genes were determined using PCR method.
Findings: Of 94 samples, 77.6% were ESBL-positive and 22.3% ESBL-negative. A total of 4.1% of the samples carried the IMP-1 gene and 43.8% the TEM gene, while 43.8% of the samples carried both genes.
Conclusion: Given that TEM and IMP-1 genes were commonly present in a large number of the resistant samples, physicians are recommended to use therapeutic measures properly, and to prescribe antibiotics rationally.
Keywords: Klebsiella pneumonia, Extended-Spectrum Betalactamase (ESBL), TEM, IMP-1, Antibiotic Resistance
Type of Study: Research Article |
Subject:
Cellular and molecular
Received: 2019/05/30 | Accepted: 2019/05/30 | Published: 2019/05/30
Received: 2019/05/30 | Accepted: 2019/05/30 | Published: 2019/05/30
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