Volume 12, Issue 45 (11-2021)
NCMBJ 2021, 12(45): 27-38 |
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Heidari S, Heidari P, Heidari B. A survey of evolutionary changes of fatty acids and storage proteins
in three Brassica species by comparative genomics method. NCMBJ 2021; 12 (45) :27-38
URL: http://ncmbjpiau.ir/article-1-1447-en.html
URL: http://ncmbjpiau.ir/article-1-1447-en.html
Department of Plant Breeding, Faculty of Agriculture, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Abstract: (2967 Views)
Aim and Background: Brassica napus has one of the most important oilseed crops in the world, which has undergone extensive genome reconstruction following an interspecies hybridization of Brassica rapa and Brassica oleracea. In order to understand the functional changes of B. napus genome during the evolution, comparison of gene expression was performed in three species of Brassica.
Materials and Methods: Seed preliminary data of three Brassica libraries were collected from the Harvard university database. To find similarities between three libraries, all EST sequences were assembled using EGassembler software. Then, all contigs were analyzed by X-blast using CLC Protein workbench software against nonredundant proteins of gene bank. IDEG6 software was used to identify genes with different expression between libraries. MapMan comparative classification tool was used to classify functional catalogs.
Results: Comparison of gene expression between the three species showed that 23 genes in 5 functional groups including fatty acids, storage proteins, amino acids, transcriptional regulation and signaling were statistically significant.
Conclusion: While B. rapa and B. oleracea encode the largest number of ESTs involved in the biosynthesis of erucic acid and linolenic acid, genome in B. napus has evolved to produce more oleic acid and linoleic acid, which may have resulted from the deletion or duplication of the genome during evolution. In addition, Cruciferin transcripts accounted for 40% of total seed storage protein transcripts. This study paves the way for further research on the genetic consequences of polyploidy during canola breeding evolution.
Materials and Methods: Seed preliminary data of three Brassica libraries were collected from the Harvard university database. To find similarities between three libraries, all EST sequences were assembled using EGassembler software. Then, all contigs were analyzed by X-blast using CLC Protein workbench software against nonredundant proteins of gene bank. IDEG6 software was used to identify genes with different expression between libraries. MapMan comparative classification tool was used to classify functional catalogs.
Results: Comparison of gene expression between the three species showed that 23 genes in 5 functional groups including fatty acids, storage proteins, amino acids, transcriptional regulation and signaling were statistically significant.
Conclusion: While B. rapa and B. oleracea encode the largest number of ESTs involved in the biosynthesis of erucic acid and linolenic acid, genome in B. napus has evolved to produce more oleic acid and linoleic acid, which may have resulted from the deletion or duplication of the genome during evolution. In addition, Cruciferin transcripts accounted for 40% of total seed storage protein transcripts. This study paves the way for further research on the genetic consequences of polyploidy during canola breeding evolution.
Type of Study: Research Article |
Subject:
Genetics
Received: 2021/09/19 | Accepted: 2021/10/30 | Published: 2021/12/22
Received: 2021/09/19 | Accepted: 2021/10/30 | Published: 2021/12/22
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