Volume 14, Issue 56 (9-2024)
NCMBJ 2024, 14(56): 43-55 |
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Heidarian F, Hojati V, Golijani Moghadam R. Comparison of A13871G polymorphism in the cytochrome P450 3A4 (CYP3A4) gene in Iranian healthy people from West Mazandaran in terms of age and sex. NCMBJ 2024; 14 (56) :43-55
URL: http://ncmbjpiau.ir/article-1-1700-en.html
URL: http://ncmbjpiau.ir/article-1-1700-en.html
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran , vida.hojati@iau.ac.ir
Abstract: (470 Views)
Aim and Background: Cytochrome P450 3A4 (CYP3A4) from the family of oxidizing enzymes is the most important enzyme in the metabolism of poisons and drugs, which is usually found in the liver and intestine, and its gene is located on chromosome 7q22.1. Since few studies have been done on the polymorphisms of this gene, this study was conducted in order to investigate the polymorphism (CYP3A4 (A13871G) in healthy male and female subjects of different ages in the west of Mazandaran.
Material and Methods: Whole blood was received. ARMS-PCR technique was used in this research, which is a simple method to detect any mutation including base changes or small deletions. Data analysis using SPSS 2016 software and the chi-square test was performed.
Results: The results showed that most of the studied subjects had the AG mutated heterozygous genotype, the frequency of which was 67.96%. There was no significant relationship between the frequency of the genotype and the age of the patients (p < 0.05). In the studied population, 50 people were female and 38 were male. In the female sex, 33 people have a heterozygous genotype with a frequency of 66%, 7 people have a dominant homozygous genotype with a frequency of 14%, 6 people have a recessive homozygous genotype with a frequency of 12%, and 4 people had no mutation with a frequency of 8%. In males, 25 people have a heterozygous genotype with a frequency of 65.78%, 3 people have a dominant homozygous genotype with a frequency of 7.89%, 5 people have a recessive homozygous genotype with a frequency of 13.15% the 5 people have no mutation with the frequency was 13.15%. Also, in both sexes, the heterozygous genotype showed the highest percentage of frequency.
Conclusion: According to the results, there is no significant relationship between genotype frequency and gender and genotype frequency and age (p < 0.05).
Material and Methods: Whole blood was received. ARMS-PCR technique was used in this research, which is a simple method to detect any mutation including base changes or small deletions. Data analysis using SPSS 2016 software and the chi-square test was performed.
Results: The results showed that most of the studied subjects had the AG mutated heterozygous genotype, the frequency of which was 67.96%. There was no significant relationship between the frequency of the genotype and the age of the patients (p < 0.05). In the studied population, 50 people were female and 38 were male. In the female sex, 33 people have a heterozygous genotype with a frequency of 66%, 7 people have a dominant homozygous genotype with a frequency of 14%, 6 people have a recessive homozygous genotype with a frequency of 12%, and 4 people had no mutation with a frequency of 8%. In males, 25 people have a heterozygous genotype with a frequency of 65.78%, 3 people have a dominant homozygous genotype with a frequency of 7.89%, 5 people have a recessive homozygous genotype with a frequency of 13.15% the 5 people have no mutation with the frequency was 13.15%. Also, in both sexes, the heterozygous genotype showed the highest percentage of frequency.
Conclusion: According to the results, there is no significant relationship between genotype frequency and gender and genotype frequency and age (p < 0.05).
Type of Study: Research Article |
Subject:
Cellular and molecular
Received: 2024/12/10 | Accepted: 2024/09/22 | Published: 2024/09/22
Received: 2024/12/10 | Accepted: 2024/09/22 | Published: 2024/09/22
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