Volume 15, Issue 58 (4-2025)
NCMBJ 2025, 15(58): 49-58 |
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Akbari S, Khalil-Moghadam S, Ghobeh M, Bikhof Torbati M, Shah Velayati A S. Investigating the antibacterial and antioxidant effects of zinc oxide nanoparticles synthesized from Arthrospira platensis with the aim of using them in wound healing. NCMBJ 2025; 15 (58) :49-58
URL: http://ncmbjpiau.ir/article-1-1740-en.html
URL: http://ncmbjpiau.ir/article-1-1740-en.html
Shabnam Akbari 
, Shiva Khalil-Moghadam , Maryam Ghobeh , Maryam Bikhof Torbati , Ashraf Sadat Shah Velayati
, Shiva Khalil-Moghadam , Maryam Ghobeh , Maryam Bikhof Torbati , Ashraf Sadat Shah Velayati
Department of Biology, Faculty of Basic Sciences, Yadegar-e-Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran , shiva.moghaddam@yahoo.com
Abstract: (250 Views)
Aim and Background: Wound care imposes a significant financial burden on the health system. One of the most important and effective factors in delaying wound healing is wound infection and high levels of reactive oxygen species (ROS). The present study examines the green synthesis of zinc oxide nanoparticles (ZnO NPs) using the aqueous extract of Arthrospira platensis.
Materials and Methods: The synthesis of ZnO nanoparticles was investigated by UV-Vis and FTIR spectroscopy and finally tested for antibacterial activity by diffusion method in agar well and antioxidant activity by DPPH assay.
Results: The results of biological synthesis using the aqueous extract of Arthrospira platensis confirmed the uniform formation. Also, the formation of effective functional groups on ZnO nanoparticles, the average size of the formed nanoparticles and the stability of suspended particles were assessed by FTIR, UV-VIS, DLS, and ZETA Potential spectra. Synthesized nanoparticles showed good antibacterial activity against bacteria involved in wound infection, such as E. coli (PTCC1399) and Pseudomonas aeruginosa (PTCC1430). The maximum diameter of the halo of non-growth of E. coli and P. aeruginosa bacteria was estimated to be 32 and 34 mm, respectively, at a concentration of 40 mg/mL. Also, the antioxidant activity of the synthesized nanoparticles showed the highest DPPH free radical inhibition with a rate of 66.60 % at a concentration of 9.25 mg/mL.
Conclusion: Using such a simple and cost-effective biological method can be a suitable alternative model for synthesizing nanomaterials on a large scale, accelerating the wound-healing process.
Materials and Methods: The synthesis of ZnO nanoparticles was investigated by UV-Vis and FTIR spectroscopy and finally tested for antibacterial activity by diffusion method in agar well and antioxidant activity by DPPH assay.
Results: The results of biological synthesis using the aqueous extract of Arthrospira platensis confirmed the uniform formation. Also, the formation of effective functional groups on ZnO nanoparticles, the average size of the formed nanoparticles and the stability of suspended particles were assessed by FTIR, UV-VIS, DLS, and ZETA Potential spectra. Synthesized nanoparticles showed good antibacterial activity against bacteria involved in wound infection, such as E. coli (PTCC1399) and Pseudomonas aeruginosa (PTCC1430). The maximum diameter of the halo of non-growth of E. coli and P. aeruginosa bacteria was estimated to be 32 and 34 mm, respectively, at a concentration of 40 mg/mL. Also, the antioxidant activity of the synthesized nanoparticles showed the highest DPPH free radical inhibition with a rate of 66.60 % at a concentration of 9.25 mg/mL.
Conclusion: Using such a simple and cost-effective biological method can be a suitable alternative model for synthesizing nanomaterials on a large scale, accelerating the wound-healing process.
Type of Study: Research Article |
Subject:
Cellular and molecular
Received: 2025/04/28 | Accepted: 2025/04/3 | Published: 2025/04/3
Received: 2025/04/28 | Accepted: 2025/04/3 | Published: 2025/04/3
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