Volume 16, Issue 61 (3-2026)
NCMBJ 2026, 16(61): 21-31 |
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Brati M, Yavari K, Aghazadeh M, Jafari H. Arsenic Calorimetric Sensor Design Using Aptasensor Based on Gold Nanoparticles. NCMBJ 2026; 16 (61) :21-31
URL: http://ncmbjpiau.ir/article-1-1796-en.html
URL: http://ncmbjpiau.ir/article-1-1796-en.html
Nuclear Science and Technology Research Institute, Tehran, Iran.
Abstract: (66 Views)
Aim and Background: Nowadays, the presence of heavy metal contamination, including arsenic, in water resources and the environment seriously threatens human health. Numerous methods have been developed for the detection of arsenic; however, they are time-consuming and expensive. The use of aptasensors attached to gold nanoparticles suspended in water is a rapid and specific method that is performed using a probe with a high degree of selectivity. In this study, a colorimetric sensor based on an aptamer–nanoparticle system was presented for the rapid and in-situ detection of arsenic in aqueous solutions.
Materials and Methods: The arsenic-specific aptamer was designed using special software’s. First, the gold nanoparticle-arsenic ion-specific aptamer complex was incubated for a specified time and room temperature. Then, arsenic samples were added to the complex at different concentrations and their sensitivity and specificity were determined using the naked eye and spectrophotometry.
Results: The sensitivity of the designed method for arsenic using the naked eye (colorimetric detection) was obtained as 0.1mM. No such color change was observed for other investigated heavy metals, indicating that this aptamer is not sensitive to structural changes induced by the other tested heavy metals and is specific only to arsenic.
Conclusion: The approaches evaluated in this study represent a rapid, inexpensive, and efficient method and have the potential to serve as a foundation for future biosensors.
Materials and Methods: The arsenic-specific aptamer was designed using special software’s. First, the gold nanoparticle-arsenic ion-specific aptamer complex was incubated for a specified time and room temperature. Then, arsenic samples were added to the complex at different concentrations and their sensitivity and specificity were determined using the naked eye and spectrophotometry.
Results: The sensitivity of the designed method for arsenic using the naked eye (colorimetric detection) was obtained as 0.1mM. No such color change was observed for other investigated heavy metals, indicating that this aptamer is not sensitive to structural changes induced by the other tested heavy metals and is specific only to arsenic.
Conclusion: The approaches evaluated in this study represent a rapid, inexpensive, and efficient method and have the potential to serve as a foundation for future biosensors.
Type of Study: Research Article |
Subject:
Biochemistry
Received: 2026/03/3 | Accepted: 2026/03/1 | Published: 2026/03/1
Received: 2026/03/3 | Accepted: 2026/03/1 | Published: 2026/03/1
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