Volume 4, Issue 16 (12-2014)
NCMBJ 2014, 4(16): 67-74 |
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Attaran Fariman G, Fatemi Y. Phylogeny of Charybdis hellerii (Decapoda, Crabs) in intertidal coasts of Chabahar based on cytochrome oxidase subunit 1 (CO1) gene sequence. NCMBJ 2014; 4 (16) :67-74
URL: http://ncmbjpiau.ir/article-1-427-en.html
URL: http://ncmbjpiau.ir/article-1-427-en.html
Chabahr Maritime University Chabahar , gilan.attaran@gmail.com
Abstract: (8722 Views)
Aim and Background:Charybdis hellerii is a well-known species in the Indian Ocean. There are many molecular researches on this species all over the word. Molecular researches on Iranian water crabs are very scarce however there are several comprehensive morphological studies. In this paper, for the first time, phylogeny of Charybdis hellerii from Iranian waters are studied.
Materials and Methods:Specimens were collected in 2013 from sandy sediments of shallow-waters in Chabahar Bay at full tide. Collections were transported to the laboratory and preserved in -20 degrees. The gill tissue was used for DNA extraction. The Hexadecyltrimethylammonium bromide (CTAB) method was used to extract DNA. Polymerase Chain Reaction (PCR) was then carried out. The sequencing alignments were conducted and phylogenic tree was obtained.
Results:The crab sample was identified as Charybdis hellerii by morphological analysis confirmed by molecular analysis. Nucleotides sequence of the cytochrome oxidase subunit 1 (CO1) gene was analyzed. The phylogenetic analysis was conducted and found that the Iranian species with other Charybdis hellerii are in a clade and support with 94% bootstrap.
Conclusion:The maximum Likelihood phylogenetic analysis showed that the Iranian species is in the Eubrachyura clad and with 94% bootstrap support is a sister group Charybdis hellerii. The molecular results were also confirmed by morphological features.
Materials and Methods:Specimens were collected in 2013 from sandy sediments of shallow-waters in Chabahar Bay at full tide. Collections were transported to the laboratory and preserved in -20 degrees. The gill tissue was used for DNA extraction. The Hexadecyltrimethylammonium bromide (CTAB) method was used to extract DNA. Polymerase Chain Reaction (PCR) was then carried out. The sequencing alignments were conducted and phylogenic tree was obtained.
Results:The crab sample was identified as Charybdis hellerii by morphological analysis confirmed by molecular analysis. Nucleotides sequence of the cytochrome oxidase subunit 1 (CO1) gene was analyzed. The phylogenetic analysis was conducted and found that the Iranian species with other Charybdis hellerii are in a clade and support with 94% bootstrap.
Conclusion:The maximum Likelihood phylogenetic analysis showed that the Iranian species is in the Eubrachyura clad and with 94% bootstrap support is a sister group Charybdis hellerii. The molecular results were also confirmed by morphological features.
Type of Study: Research Article |
Subject:
Genetics
Received: 2013/12/24 | Accepted: 2014/12/27 | Published: 2014/12/27
Received: 2013/12/24 | Accepted: 2014/12/27 | Published: 2014/12/27
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