Volume 15, Issue 57 (12-2024)
NCMBJ 2024, 15(57): 33-42 |
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Derakhshan Y, Babapour E, Jamalifar H. Preparing extract from baker's yeast and investigating the ability of bacteria to grow on it. NCMBJ 2024; 15 (57) :33-42
URL: http://ncmbjpiau.ir/article-1-1716-en.html
URL: http://ncmbjpiau.ir/article-1-1716-en.html
Biotechnology Research Center, Karaj Branch, Islamic Azad University, Karaj, Iran , e_babapoor@yahoo.com
Abstract: (470 Views)
Background and Aim: Saccharomyces cerevisiae, known as baker's yeast, is used to produce yeast extract. The mentioned extract has wide applications in medicine, food industry, and most importantly as one of the main sources of food for microbiology cultures because it contains vitamins and substances needed for growth. The aim of this study was to develop a simple and profitable process for the production of yeast extract to prepare bacterial culture medium.
Materials and Methods: First, yeast extract was prepared from baker's yeast based on a simple protocol and as a result of changing temperature and high pressure, and after centrifugation, its liquid phase was converted into a solid form by means of a spray dryer, and then with a certain amount of agar from that microbial culture medium. It was prepared and then Gram-positive bacteria (Staphylococcus aureus ATCC 25923) and Gram-negative bacteria (Escherichia coli ATCC 25922) were used to check the ability and quality of bacteria to grow on it. To compare the growth rate of bacteria, standard culture media prepared from Merck (Germany) yeast extract and Mirmedia (Iran) yeast extract, Merck (Germany) tryptic soy agar was used.
Results: The obtained results indicate that the yeast extract from this study, as the only nutrient source for bacterial growth, is sufficiently available and the microorganisms are able to grow in that environment. Also, competitive competition with commercial yeast extracts can be used in the preparation of culture medium.
Conclusion: Considering the quality and rate of bacterial growth on the culture medium prepared in this study, and considering the wide application of yeast extract in the industry, and considering that no chemicals and enzymes were used in its preparation, the preparation of yeast extract in this way is of acceptable quality and affordable.
Materials and Methods: First, yeast extract was prepared from baker's yeast based on a simple protocol and as a result of changing temperature and high pressure, and after centrifugation, its liquid phase was converted into a solid form by means of a spray dryer, and then with a certain amount of agar from that microbial culture medium. It was prepared and then Gram-positive bacteria (Staphylococcus aureus ATCC 25923) and Gram-negative bacteria (Escherichia coli ATCC 25922) were used to check the ability and quality of bacteria to grow on it. To compare the growth rate of bacteria, standard culture media prepared from Merck (Germany) yeast extract and Mirmedia (Iran) yeast extract, Merck (Germany) tryptic soy agar was used.
Results: The obtained results indicate that the yeast extract from this study, as the only nutrient source for bacterial growth, is sufficiently available and the microorganisms are able to grow in that environment. Also, competitive competition with commercial yeast extracts can be used in the preparation of culture medium.
Conclusion: Considering the quality and rate of bacterial growth on the culture medium prepared in this study, and considering the wide application of yeast extract in the industry, and considering that no chemicals and enzymes were used in its preparation, the preparation of yeast extract in this way is of acceptable quality and affordable.
Type of Study: Research Article |
Subject:
Cellular and molecular
Received: 2025/02/19 | Accepted: 2024/12/21 | Published: 2024/12/21
Received: 2025/02/19 | Accepted: 2024/12/21 | Published: 2024/12/21
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